Contact an Account ManagerDetermination of Hypericin and Pseudohypericin from Hypericum
Summary
Type: HPLC
Application #: 119400
Catalog#: 760100.30 - MN HPLC Column, Nucleodur C18 Gravity, 110A, 5um, 3x125mm
Detection: VIS 590 nm
Conditions: Injection: 20 μl
Eluents:
A: Acetonitril
B: 0,1 M Ammoniumacetat in water
C: Methanol/Ethylacetat 50:50 (V/V)
Gradient:
Time A / B / C
0-15 min 40 / 60 / 0
15-20 min 40 / 35 / 25
20-25 min 60 / 0 / 40
25-26 min 100 / 0 / 0
26-35 min 40 / 60 / 0
35 - min 40 / 60 / 0
Flow rate: 0.6 ml/min
Temperature: 30°C
Authors: Leiter G
Source: SALUS Haus Dr med Otto Greither Nachf GmbH & Co KG
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